Analysis of the related factors of subfoveal choroidal thickness in children with low to moderate myopia / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To explore the related factors that affect the subfoveal choroidal thickness(SFCT)of children with low to moderate myopia. <p>METHODS: A cross-sectional study. We selected children aged 6-12 who were diagnosed as myopia in the Department of Ophthalmology of the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University from October to December 2020, according to the spherical equivalent refraction(SER), 60 patients(60 eyes)with low myopia of -0.75 to -3.00D and 50 patients(50 eyes)with moderate myopia of -3.25 to -6.00D, which was obtained by cycloplegic refraction. All participants conducted ophthalmic examination, including measurements of SFCT, subfoveal retinal thickness(SFRT), axial length(AL), and parameters of ocular accommodation. Children's age, sex, parental myopia, height and weight were collected. The relationship between SFCT and various factors was investigated.<p>RESULTS: The average SFCT of the low and moderate myopia were 273.30±44.93 and 237.16±48.56μm(<i>P</i><0.01), respectively. Pearson's correlation analysis showed that SFCT was statistically related to SER, AL and height(<i>r</i>=0.35, -0.45, -0.19, all <i>P</i><0.05), and no statistical correlation with other factors(all <i>P</i>>0.05). Multiple linear regression showed that SFCT became thinner as AL became longer(<i>B</i>= -19.87, <i>P</i><0.01). <p>CONCLUSION:SFCT in children with low to moderate myopia was statistically related to SER, AL and height, but it was not notably related to other factors. AL was the most significant factor affecting SFCT.

Relationship between Small-world Network and Cognitive Impairment for Patients with White Matter Lesions Based On Diffusion Tensor Imaging / 中国康复理论与实践

Objective:To explore the relationship between small-worldness of brain network and cognitive impairment in patients with white matter lesions (WMLs) based on diffusion tensor imaging (DTI). Methods:From January, 2016 to December, 2017, 46 WMLs patients and 36 controls matched genders, ages and education levels from Beijing Tiantan Hospital were screened with DTI. The patients were divided into vascular cognitive impairment non-dementia (VCIND) and vascular dementia (VaD) groups according to the results of cognitive assessments. The brain structure network was created based on DTI data, and the topological properties of the whole-brain small-world network were calculated, and the correlation between the small-worldness and the severity of cognitive impairment was analyzed. Results:The global efficiency, local efficiency, shortest path length and clustering coefficient were different between the patients and the controls (F > 3.252, P < 0.05), as well as the properties of the small-world network, λ, γ and σ (F > 7.378, P < 0.01). The λ, γ and σ were correlated with the total score of Montreal Cognitive Assessment (|r| > 0.402, P < 0.05). Conclusion:The brain structure network is small-world network for patients with WMLs, and the decrease of small-world properties may relate to the cognitive impairment.

Change laws of water absorption in Chinese herbal pieces and prediction model of relative density for Chinese medicine decoction / 中国中药杂志

This study aims to explore the change laws of water absorption in Chinese herbal pieces and establish the prediction model of relative density for Chinese medicine compound decoction. Firstly, fitted equations of water absorption and decocting time was established by observing the change laws of water absorption in 36 kinds of Chinese herbal pieces in 12 groups(according to the drug-parts) with decocting time. The r value of the mineral group and other type group was 0.691 2 and 0.663 3, respectively. The r value of the remaining 10 groups was 0.802 2-0.925 4. All P values were less than 0.05(n=21). The formula of the amount of water added was optimized by combining the fitted equations with determined water absorption, and the liquid yield could be controlled in a range of 100%±10%. Secondly, it was determined that the liquid density tester could be used for the rapid determination of relative density of Chinese medicine decoction after methodological study and comparison with the pycnometer method. The linear regression equation between the corrected relative density(y) and extraction ratio(%, x) was built by measuring and analyzing the related parameters such as liquid yield, relative density and extraction ratio in 46 kinds of Chinese herbal pieces. The established equation was y=0.041 3x+1.003 7, r=0.930 9(P <0.01, n=46), with linear range of 1.94%-65.75%. Based on this, the prototype model for predicting relative density of Chinese medicine decoction was established, and the relative densities of 8 Chinese medicine decoctions were within the prediction interval of this model in verification. This study lays a foundation for database construction of Chinese medicine decoction, implementation of personalized decocting mode and rapid quality control of Chinese medicine decoction.

Analysis of the Success Rate and Reliability of Fibroscan in Screening Fatty Liver / 中山大学学报(医学科学版)

@#【Objective】Fibroscan has been recommended by multiple clinical guidelines as the first choice among the non-invasive tools for evaluating hepatic steatosis and liver stiffness. The study herein assessed the factors related to the success rate and reliability of the measurement.【Methods】A total of 788 volunteers from Year 2018′ s staff physical checkup in The First Affiliated Hospital of Sun Yat- sen University were recruited. Relationship between age ，gender， body mass index ，waist circumference of the subjects ，and the operator′s experience ，and their effect on success rate and reliability were analyzed.【Results】had a higher detection rate for fatty liver as compared with ultrasound（55.9% vs.39.9% ，P<0.001）. Detection failure and unreliable measurements occurred in only 1.4% of all the participants. Success rate was not associated with gender ，but decreased significantly with increased age ，body mass index and waist circumference （P<0.05）. LSM IQR/Median increased accordingly ，thus reduced the reliability of the measurement. With accumulated number of measurements by the same operator，so did the success rate and reliability of the results. An operator with more than 300 measurements could be deemed as experienced operator.【Conclusions】The success rate and reliability of the measurement was affected by increased age ，body mass index ，waist circumference and operator′ s experience. Fixed operator could also achieve very high success rate and reliable measurement using Fibroscan after short- term training.

Resting-state Neural Networks and Granger Causal Connectivity for Patients with Cognitive Impairment Associated with Leukoaraiosis / 中国康复理论与实践

Objective:To compare the difference in resting state networks among leukoaraiosis (LA) patients with or without mild cognitive impairment, and healthy controls, as well as the functional connectivity under Granger causality analysis (GCA). Methods:Subjects aged 40 to 80 years, including 34 LA-MCI patients, 15 LA patients with normal cognition and 33 healthy controls, accepted resting-state functional magnetic resonance imaging. Independent component analysis was used to separate functional brain networks, and difference of activation was determined with two sample t-test. GCA was used to analyze effective connectivity of these functional networks. Results:Eight resting state networks were obtained, including default mode network, motor network, medial visual network, lateral visual network, right-memory network, left-memory network, auditory network and executive network. Activation was different among three groups. Effective connectivity of RSNs was also different among three groups. Conclusion:Components of the resting state networks keep changing as LA progressing. Activation decreases as patients' cognition impaired. The direction and strength of connections remodel.

Clinical, Laboratory and Imaging Features of High Altitude Pulmonary Edema in Tibetan Plateau / 中国医学科学杂志(英文版)

Objective To analyze characteristics of high altitude pulmonary edema (HAPE) in Chinese patients.Methods We performed a retrospective study of 98 patients with HAPE. We reviewed the medical records and summarized the clinical, laboratory and imaging characteristics of these cases, and compared the results on admission with those determined before discharge.Results Forty-eight (49.0%) patients developed HAPE at the altitude of 2800 m to 3000 m. Ninty-five (96.9%) patients were man. Moist rales were audible from the both lungs, and moist rales over the right lung were clearer than those over the left lung in fourteen patients. The white blood cells [(12.83±5.55) versus (8.95±3.23) ×10 /L, P=0.001)] as well as neutrophil counts [(11.34±3.81) versus (7.49±2.83)×10 /L, P=0.001)] were higher, whereas the counts of other subsets of white blood cells were lower on admission than those after recovery (all P<0.05). Serum levels of alkaline phosphatase (115.8±37.6 versus 85.7±32.4 mmol/L, P=0.020), cholinesterase (7226.2±1631.8 versus 6285.3±1693.3 mmol/L, P=0.040), creatinine (85.2±17.1 versus75.1±12.8 mmol/L, P=0.021), uric acid (401.9±114.2 versus 326.0±154.3 mmol/L, P=0.041), and uric glucose (7.20±1.10 versus 5.51±1.11 mmol/L, P=0.001) were higher, but carbondioxide combining power (COCP, 26.7±4.4 versus 28.9±4.5 mmol/L, P=0.042) and serous calcium (2.32±0.13 versus 2.41±0.10 mmol/L, P=0.006) were lower on admission. Arterial blood gas results showed hypoxemia and respiratory alkalosis on admission. Conclusions In the present research, men were more susceptible to HAPE than women, and in the process of HAPE, the lesions of the right lung were more serious than those of the left lung. Some indicators of routine blood test and blood biochemistry of HAPE patients changed.

Impact of Cholinergic Pathway Damage on Cognitive Impairment Caused by Cerebral White Matter Lesions / 中国康复理论与实践

Objective To investigate the relationship between cholinergic pathway damage and the executive dysfunction of patients with different degrees of cognitive impairment caused by cerebral white matter lesions(WML). Methods From March,2016 to December,2017,115 patients were recruited,whose characteristics,such as age,gender, education,and history of hypertension,diabetes and stroke were recorded.According to the T2-weighted MRI,80 patients were defined as WML.WML patients were divided into cognitively normal(CN)group(n=41),vascular cognitive impairment of none dementia(VCIND)group(n=21)and vascular dementia(VaD)group(n=18)ac-cording to the result of Montreal Cognitive Assessment(MoCA)and Clinical Dementia Rating(CDR).Other 35 cases without WML and cognitive impairment were as control group.WML under MRI were evaluated with Cho-linergic Pathways Hyperintensities Scale(CHIPS).All the WML patients were assessed with Stroop Color-Word Test,Trail Making Test, Symbol Digital Modalities Test, and Verbal Fluence Test.The correlation between the scores of CHIPS and the executive tests were analysed. Results There was no significant difference in age, gender, level of education, and cardiovascular disease risk factors among four groups(P>0.05),but there were significant differences in scores of MoCA and CHIPS(F>25.781,P<0.001),while the score of MoCA was the least(P<0.01)and the scores of CHIPS were the most in VaD group (P<0.001).The CHIPS scores of left and bilateral hemisphere negatively correlated with all the scores of execu-tive tests(P<0.05),while that of the right hemisphere just correlated with the scores of some executive tests(P<0.05). Conclusion For cognitive impairment after WML,cholinergic pathway damage may relate with the executive function impairment,especially the damage in left cerebral hemisphere.

Advance in Default Mode Network,Salience Network and Central Executive Network about Cognitive Function in Old Persons(review) / 中国康复理论与实践

@#A lot of researches indicate the relationship between cognitive impairment and functional connectivity of default mode network,salience network and central executive network.The changes of networks are various in different cognitive dys-function.It is important to apply resting state functional magnetic resonance in the old adults with cognitive dysfunction.

Resting-state Network of Brain in Leukoaraiosis Patients: A Magnetic Resonance Imaging Study / 中国康复理论与实践

@#Objective To explore the diversity of resting-state network of brain between the patients with leukoaraiosis and the healthy people. Methods 31 patients with leukoaraiosis (patients) and 27 healthy persons (controls) were checked with resting-state functional magnetic resonance imaging (rs-fMRI), and analyzed with the independent component analysis (ICA) to explore the resting-state functional brain network. Results The resting-state brain network was found in both the patients and the controls, which was coincident with the previous studies. The active areas were the same in both groups, and the activation was weaken in the patients than in the controls, especially in quadrate gyri, posterior cingulate cortex, superior temporal gyrus, superior parietal gyrus, anterior central gyrus, post central gyrus, insula and prefrontal cortex. Conclusion There is a significant diversity of resting-state network of brain between the patients with leukoaraiosis and healthy people in the activation of active areas.

Establishment of an aromatase inhibitor letrozole-resistant breast cancer cell model / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To establish a human breast cancer MCF-7 cell model stably overexpressing the aromatase gene (MCF-7-aromatase) and aromatase inhibitor letrozole-resistant MCF-7 cell model (MCF-7-LR).</p><p><b>METHODS</b>We utilized the lentivirus-mediated gene transfer approach to establish MCF-7-aromatase cell and MCF-7 cell model stably overexpressing green fluorescent protein (GFP) (MCF-7-GFP). The expression of aromatase in the MCF-7-aromatase and MCF-7-GFP cells was determined by reverse transcription polymerase chain reaction (RT-PCR), real time quantitative PCR (RT-qPCR), Western blot and immunoprecipitation (IP) assay. The proliferative ability in vitro of MCF-7-aromatase and MCF-7-GFP cells treated with testostorone and β-estradiol (E2) was determined by WST-1 cell proliferation assay. The proliferative ability of MCF-7-aromatase cells treated with letrozole was determined by WST-1 assay. The half maximal inhibitory concentration (IC50 value) for letrozole was calculated from the nonlinear regression line of the plot of cell viability (percentage of control) versus letrozole concentration using Graphpad Prism software. MCF-7-aromatase cells were continuously cultured in the presence of testosterone and letrozole, thus letrozole-resistant MCF-7-LR cells were obtained. WST-1 assay was performed to determine their chemoresistance to letrozole.</p><p><b>RESULTS</b>RT-PCR and RT-qPCR results revealed that the mRNA expression of aromatase was significantly increased in the MCF-7-aromatase cells compared with that in the MCF-7-GFP cells. Both Western blot and IP assays showed that the expression of aromatase protein was drastically increased in the MCF-7-aromatase cells, compared with that in the MCF-7-GFP cells. WST-1 assay showed that the cell proliferation rate of MCF-7-aromatase cells treated with 1 and 10 nmol/L testosterone was 1.43- and 1.53-fold higher than that of the control cells, respectively. The proliferation rate of MCF-7-aromatase cells treated with 1 and 10 nmol/L E2 was 1.41- and 1.55-fold higher than that of the control cells, respectively. In contrast, the proliferation rate of MCF-7-GFP cells treated with 10 nmol/L testosterone was 1.12-fold higher than that of the control cells, and the proliferation rate of MCF-7-GFP cells treated with 1 and 10 nmol/L E2 was 1.41- and 1.51-fold higher than that of the control cells, respectively. Letrozole treatment significantly inhibited the testosterone-induced proliferation ability of MCF-7-aromatase cells in a dose-dependent manner and the IC50 value was 5.3 nmol/L. In contrast, letrozole treatment showed no inhibitory effect on the proliferative ability of MCF-7-LR cells and the IC50 value was >1000 nmol/L.</p><p><b>CONCLUSIONS</b>MCF-7-aromatase and MCF-7-LR cells exhibit different response to letrozole treatment, which provides an important basis for further investigating the mechanism of letrozole resistance.</p>

Regional homogeneity analysis on acupoint specificity with resting-state functional magnetic resonance imaging / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The mechanism of acupuncture analgesia in craniotomy has been widely studied. However, the theoretical basis for selection of acupoints has not been examined. In this study, we used the regional homogeneity method blood oxygen level-dependent (BOLD) signals to determine changes in brain activity in response to transcutaneous electrical stimulation on acupoints and non-acupoints in resting state functional magnetic resonance imaging (fMRI).</p><p><b>METHODS</b>Twelve healthy volunteers were enrolled in this study. BOLD fMRI scanning of the brain was performed for 306 seconds before and 30 minutes after transcutaneous electrical stimulation on acupoints UB63 (Jinmen), LV3 (Tai chong), ST36 (Zusanli), and GB40 (Qiuxu). The procedure was repeated after one week with stimulation on non-acupoints (one was 9 above BL67, the second was 12 above BL67 (Kunlun), the third was 7 above KI3, and the fourth was 10 above KI3 (Taixi)).</p><p><b>RESULTS</b>The regional homogeneity in the acupoint group was increased in the left thalamus, caudate, putamen, lentiform nucleus (BA19, 30, 39), postcentral gyrus, precentral gyrus (BA3, 4, 30, 32), calcarine fissure, middle temporal gyrus (BA30), right superior temporal gyrus, inferior temporal gyrus (BA38), cuneus, and precuneus (BA7, 19) when compared to the non-acupoint group. The regional homogeneity of the acupoint group was decreased in the left cerebellum posterior lobe, middle frontal gyrus (BA10), double-side precuneus (BA7), and the postcentral gyrus (BA40).</p><p><b>CONCLUSIONS</b>The brain region activated following acupoint stimulation is the ipsilateral pain-related brain region, which may relate to the therapeutic effect of acupuncture on pain relief. Further acupoint stimulation causes different central nervous responses compared to non-acupoint stimulation.</p>

Effects of baicalin against oxidative stress injury of SH-SY5Y cells by up-regulating SIRT1 / 药学学报

This study is to investigate the effect of baicalin (BL) against oxidative injury stress of SH-SY5Y cells induced by H2O2 and the possible mechanism. SH-SY5Y cells were pre-incubated with baicalin (25, 50, and 100 micromol x L(-1)) for 12 h prior to exposure to H2O2 (150 micromol x L(-1)) for 24 h. The viability of SH-SY5Y cells was measured by MTT assay. The contents of LDH and NO were determined. The percentage of apoptotic cells was assessed by flow cytometry (FCM). The content of Caspase-3 was tested by immunofluorescence histochemical method. BL at 50 and 100 micromol x L(-1) separately increased the cell viability and up-regulated SIRT1, reduced the contents of LDH, NO, Caspase-3 and the apoptotic percentage of SH-SY5Y cells. This study results suggest that baicalin could inhibit the H2O2-induced neuronal apoptosis. The further mechanism studies show that baicalin inhibit apoptosis via reducing Caspase-3 expression and up-regulating SIRT1.

Study on pancreas of type 1 diabetic mice induced by MLD-STZ using proteomics / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To get a better understanding of the mechanisms underlying type 1 diabetes mellitus, the differentially expressed pancreatic proteins from multiple low-dose streptozotocin (MLD-SIZ) mouse and normal mouse were analyzed and compared.</p><p><b>METHODS</b>20 male rats were separated into 2 groups (n=10): model mice treated with MLD-STZ and normal mice,differences of pancreatic proteome among in the two groups of mice, were analyzed by two dimensional polyacryamide gel electrophoresis (2DE). Protein quantification was analyzed and the differentially expressed spots were identified using mass spectrometry and MASCOT database searching.</p><p><b>RESULTS</b>Compared with control group, 23 proteins had changed significantly in the model group, 8 proteins expression were up-regulated, 15 proteins expressions down-regulated significantly. Using MALDI-TOF-MS, 15 proteins with significant change were identified by peptide fingerprinting map and the results were searched in MASCOT database. The function analyzed showed that proteins with change were associated with metabolic, anti-oxidant, structural, catalytic enzymes and chaperone, et al.</p><p><b>CONCLUSION</b>Type 1 diabetes is probably exerted via multi-target and multi-path mechanism. The proteins with significant change are newly target for type 1 diabetes early diagnosis and treatment.</p>

Prediction of neural tube defect using support vector machine / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To predict neural tube birth defect (NTD) using support vector machine (SVM).</p><p><b>METHOD</b>The dataset in the pilot area was divided into non overlaid training set and testing set. SVM was trained using the training set and the trained SVM was then used to predict the classification of NTD.</p><p><b>RESULT</b>NTD rate was predicted at village level in the pilot area. The accuracy of the prediction was 71.50% for the training dataset and 68.57% for the test dataset respectively.</p><p><b>CONCLUSION</b>Results from this study have shown that SVM is applicable to the prediction of NTD.</p>

Protective effect of extract of Apocynum venetum on kidneys of streptozotocin-induced diabetic rats / 药学学报

The aim of this study is to investigate the effects and mechanism of extract of Apocynum venetum (AV) on kidneys of streptozotocin-induced diabetic rats. Diabetes mellitus (DM) was induced in rats by intraperitoneal injection of streptozotocin (STZ). The indexes of the blood glucose, renal function and oxidative stress were observed. The DM rats were administrated with the AV for 8 weeks, the above-mentioned indexes were detected. The blood glucose level, BUN, 24 h urine protein excretion, urine volume, renal index, renal cortex's MDA level in model groups all increased significantly. Renal cortex's SOD and GSH activities decreased significantly compared with the normal control group (P < 0.05). The above-mentioned indexes were significantly improved by the AV treatment (P < 0.05). AV have protective effects on renal function of kidneys of streptozotocin-induced diabetic rats, and maybe via inhibition of the renal oxidative stress.

Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation / 药学学报

The aim of this study is to investigate the effect of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation. Duck hepatitis B virus (DHBV) infection model and normal mouse spleen lymphocyte were used to evaluate the anti-HBV and immunoregulation effects. The DHBV-DNA of serum was detected at different time points by using serum DOT-BLOT hybridization. Polymerase chain reaction (PCR) was used for the determination of nuclear covalent closed circular DNA (cccDNA). Cytokine secretion was determined by ELISA method. DHBV-DNA were inhibited by hyperin (25 or 50 mg x kg(-1)), while cccDNA of liver could be eliminated efficiently by hyperin (25 or 50 mg x kg(-1), P < 0.05, P < 0.01). The T helper 1 effector cytokine was markedly enhanced by hyperin (25 or 50 microg x mL(-1), P < 0.01). In conclusion, hyperin has anti-HBV activity via multiple targets and pathways, and cccDNA may be one of the important targets.

Stability of physical state on compound hawthorn dropping pills / 中国中药杂志

<p><b>OBJECTIVE</b>To evaluate the stability of physical state with accelerate test and dropping in process before and after on compound hawthorn dropping pills.</p><p><b>METHOD</b>Scanning electron microscope, TG-DTA, FT-IR and XRD were used.</p><p><b>RESULT</b>The active components presented amorphous, tiny crystal and molecular state in dropping pills, and it had no obvious reaction between PEG 4000 and active components. With time prolonging, a little of active components changed from amorphous state to tiny crystal or molecular state.</p><p><b>CONCLUSION</b>Solid dispersion improved the stability and dissolution of compound hawthorn dropping pills.</p>

Study on the mechanism of acupuncture at Daling (PC 7) for mental diseases by fMRI / 中国针灸

<p><b>OBJECTIVE</b>To probe into the central nervous mechanism of acupuncture at Daling (PC 7) treating mental diseases by fMRI.</p><p><b>METHODS</b>Eight healthy volunteers were enrolled in the study. Acupuncture stimulation was given at Daling (PC 7) in each subject with a stimulating pattern, "rest-stimulation-rest-stimulation-rest". The fMRI data were analyzed with SPM 2 software package.</p><p><b>RESULTS</b>The activating areas of acupuncture at Daling (PC 7) were mainly on Brodmann 46/47/44/9 areas of inferior frontal gyrus, Brodmann 6 area of middle frontal gyrus, Brodmann 22 area of superior temporal gyrus and Brodmann 40 area of postcentral gyrus and inferior parietal lobule.</p><p><b>CONCLUSION</b>Pathogenetic factors of mental diseases are very complicated, damange or hypofunction or inadequate activation of frontal lobe and temporal lobe are closely related with mental diseases. Therefore, activating the frontal and temporal lobe possibly is one of the mechanisms of acupuncture at Daling (PC 7) treating mental diseases.</p>

Cloning and sequence analysis of the Meq gene of 4 Marek's disease virus isolates from China / 病毒学报

Earlier studies have determined that the repeat regions of oncogenic serotype 1 MDV (Marek's disease virus) encode a basic leucine zipper protein, Meq, which structurally resembles the Jun/Fos family of transcriptional activators. Meq has been suggested as the MDV-associated oncogene. In this paper, based on the published sequence of Meq gene of GA strain of MDV, a pair of primers were designed and synthesized. Meq gene ORF (Open reading frame) of the four Chinese local MDV isolates, the reference strain J-1 and the vaccine strain 814 were amplified by using polymerase chain reaction(PCR). Then the PCR products were cloned and sequenced respectively. The results of sequence comparison indicated that the sequences of Meq gene in different strains are relatively conserved and homology of the amino acid sequences is 96.5%-99.7%. The proline-rich repeats of Meq gene of four MDV isolates have site mutations, and it is related to MDV's virulence. Two unique site mutations appear in Meq gene of Chinese local MDV isolates, but they aren't present in Meq gene of the published MDV strains from abroad and the early domestic strains. It seems that some regularities exist between such mutations in four Chinese local MDV isolates and the virulence of MDV, but the regularities need further research.

Investigation of Pathologic Mechanism of Motor Aphasia: An Analysis of 25 fMRI Cases / 中国康复理论与实践

@#ObjectiveTo investigate the pathologic mechanism of motor aphasia.Methods25 patients with cerebral infarction and motor aphasia were examined by magnetic resonance spectroscopy (MRS) and perfusion weighted imaging (PWI) at Broca's areas, the results were compared with that of the mirror side.ResultsMRS showed that the N-acetyl aspartate, choline in Broca's areas reduced than that of the mirror side ( P<0.05); while PWI showed that the regional cerebral blood volume and regional cerebral blood flow of damaged Broca's areas decreased significantly than that of the right hemisphere ( P<0.01). Mean transit time and time to peak of damaged Broca's areas prolonged than that of mirror side ( P<0.05).ConclusionHypoperfusion and hypometabolism revealed in Broca's areas of patients with motor aphasia may be one of the mechanisms of motor aphasia.